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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/07/2020 |
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Actualizado : |
24/02/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MARCHI, P. M.; CARVALHO, I. R; DINI, M.; SZARESKI, V. J.; PELEGRIN, A. J. DE; PEREIRA, I. S.; ANTUNES, L. E. C. |
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Afiliación : |
P. M. MARCHI, Empresa Brasileira de Pesquisa Agropecuária, Estação Experimental de Fruticultura de Clima Temperado, Pelotas, RS, Brazil; I.R. CARVALHO, Universidade Regional do Noroeste do Estado do Rio Grande do Sul, Ijuí, RS, Brazil; MAXIMILIANO ANTONIO DINI VIÑOLY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; V.J. SZARESKI, Universidade Federal de Pelotas, Pelotas, RS, Brazil; A.J. DE PELEGRIN, Universidade Federal de Pelotas, Pelotas, RS, Brazil; I.S. PEREIRA, Empresa Brasileira de Pesquisa Agropecuária, Estação Experimental de Fruticultura de Clima Temperado, Pelotas, RS, Brazil; L.E.C. ANTUNES, Empresa Brasileira de Pesquisa Agropecuária, Estação Experimental de Fruticultura de Clima Temperado, Pelotas, RS, Brazil. |
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Título : |
Canonical correlations among morphological and yield traits in primocane-fruiting raspberries. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Genetics and Molecular Research, 30 June 2020, Volume 19, Issue 2, Article number gmr18500, Pages 1-7. OPEN ACCESS. DOI: http://dx.doi.org/10.4238/gmr18500 |
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ISSN : |
1676-5680 |
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DOI : |
10.4238/gmr18500 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received October 11, 2019; Accepted May 28, 2020; Published June 30, 2020.
Corresponding author: Carvalho, I.R.; Universidade Regional do Noroeste do Estado do Rio Grande do Sul, Ijuí, RS, Brazil; email:carvalho.irc@gmail.com |
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Contenido : |
ABSTRACT.
Raspberry (Rubus idaeus) is an economically important crop, and its cultivation has expanded recently across temperate and subtropical regions of Brazil. There is no brazilian raspberry cultivar so far. Therefore, the indirect selection can be an useful tool to help during the breeding process. We evaluated linear and canonical correlations in primocane-fruiting raspberry cultivars in 2013 and 2014, in a completely randomized experimental design, with eight replications. The genotypes ?Alemãzinha?, ?Heritage? and ?Fallgold? were evaluated during two harvesting seasons, autumn and spring. Canonical groups were established among morphological (Group 1) and yield (Group 2) traits. In Group 1, plant yield and fruit weight were evaluated; in Group 2 the productive period, plant height, stem diameter, dry mass of pruning and plant leaf area were assessed. The linear and canonical correlations among morphological, phenological, and yield traits varied according to harvesting season. The phenological and morphological traits had greater linear correlation with yield traits in autumn than in spring. Based on our findings, to obtain more productive raspberry genotypes, plant leaf area and dry mass of pruning should be prioritized in the indirect selection. © FUNPEC-RP. |
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Palabras claves : |
Indirect selection; Rubus idaeus; Small fruit breeding. |
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Asunto categoría : |
F01 Cultivo
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15062/1/Marchi-et-al.-Canonical-correlations-raspberries-GMR-2020.pdf
http://www.funpecrp.com.br/gmr/articles/year2020/vol19-2/pdf/gmr18500_-_canonical-correlations-among-morphological-and-yield-traits-primocane-fruiting-raspberries.pdf
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Marc : |
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005 2021-02-24
008 2020 bl uuuu u00u1 u #d
022 $a1676-5680
024 7 $a10.4238/gmr18500$2DOI
100 1 $aMARCHI, P. M.
245 $aCanonical correlations among morphological and yield traits in primocane-fruiting raspberries.$h[electronic resource]
260 $c2020
500 $aArticle history: Received October 11, 2019; Accepted May 28, 2020; Published June 30, 2020. Corresponding author: Carvalho, I.R.; Universidade Regional do Noroeste do Estado do Rio Grande do Sul, Ijuí, RS, Brazil; email:carvalho.irc@gmail.com
520 $aABSTRACT. Raspberry (Rubus idaeus) is an economically important crop, and its cultivation has expanded recently across temperate and subtropical regions of Brazil. There is no brazilian raspberry cultivar so far. Therefore, the indirect selection can be an useful tool to help during the breeding process. We evaluated linear and canonical correlations in primocane-fruiting raspberry cultivars in 2013 and 2014, in a completely randomized experimental design, with eight replications. The genotypes ?Alemãzinha?, ?Heritage? and ?Fallgold? were evaluated during two harvesting seasons, autumn and spring. Canonical groups were established among morphological (Group 1) and yield (Group 2) traits. In Group 1, plant yield and fruit weight were evaluated; in Group 2 the productive period, plant height, stem diameter, dry mass of pruning and plant leaf area were assessed. The linear and canonical correlations among morphological, phenological, and yield traits varied according to harvesting season. The phenological and morphological traits had greater linear correlation with yield traits in autumn than in spring. Based on our findings, to obtain more productive raspberry genotypes, plant leaf area and dry mass of pruning should be prioritized in the indirect selection. © FUNPEC-RP.
653 $aIndirect selection
653 $aRubus idaeus
653 $aSmall fruit breeding
700 1 $aCARVALHO, I. R
700 1 $aDINI, M.
700 1 $aSZARESKI, V. J.
700 1 $aPELEGRIN, A. J. DE
700 1 $aPEREIRA, I. S.
700 1 $aANTUNES, L. E. C.
773 $tGenetics and Molecular Research, 30 June 2020, Volume 19, Issue 2, Article number gmr18500, Pages 1-7. OPEN ACCESS. DOI: http://dx.doi.org/10.4238/gmr18500
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
24/10/2023 |
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Actualizado : |
24/10/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
NOVAS, R.; BASIKA, T.; WILLIAMSON, M. E.; FRESIA, P.; MENCHACA, A.; SCOTT, M. J. |
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Afiliación : |
ROSSINA NOVAS, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; MEGAN E. WILLIAMSON, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MAXWELL J. SCOTT, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States. |
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Título : |
Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Insect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS. |
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ISSN : |
0962-1075 (print); 1365-2583 (electronic). |
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DOI : |
10.1111/imb.12875 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. -- |
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Contenido : |
The New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. |
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Palabras claves : |
CRISPR/Cas9; Genome editing; Partnership for the goals - Goal 17; Pest control; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Screwworm; Sustainable Development Goals (SDGs); U6 promoter. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
https://resjournals.onlinelibrary.wiley.com/doi/pdfdirect/10.1111/imb.12875
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Marc : |
LEADER 02928naa a2200313 a 4500
001 1064356
005 2023-10-24
008 2023 bl uuuu u00u1 u #d
022 $a0962-1075 (print); 1365-2583 (electronic).
024 7 $a10.1111/imb.12875$2DOI
100 1 $aNOVAS, R.
245 $aIdentification and functional analysis of Cochliomyia hominivorax U6 gene promoters.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. --
520 $aThe New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society.
653 $aCRISPR/Cas9
653 $aGenome editing
653 $aPartnership for the goals - Goal 17
653 $aPest control
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aScrewworm
653 $aSustainable Development Goals (SDGs)
653 $aU6 promoter
700 1 $aBASIKA, T.
700 1 $aWILLIAMSON, M. E.
700 1 $aFRESIA, P.
700 1 $aMENCHACA, A.
700 1 $aSCOTT, M. J.
773 $tInsect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS.
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